Mathematical Modelling and Variable Structure Control Systems for Fed-Batch Fermentation of Escherichia coli

Intercriteria Analysis for Identification of Escherichia Coli Fed- Batch Mathematical Model

Escherichia coli produces insulin, interferons, growth factors, exoand endoproducts, e.g. enzymes, etc. In this paper we present a new approach for multicriteria decision making – InterCriteria Analysis to mathematical modelling of a fermentation process. It is based on the apparatus of index matrices and intuitionistic fuzzy sets. The approach for multicriteria analysis makes it possible to co...

Implementation of a Specific Rate Controller in a Fed-batch E. Coli Fermentation

The specific growth rate is one of the most important process variables characterizing the state of microorganisms during fermentations mainly because the biosynthesis of many products of interest is often related with the values assumed by this parameter. In the particular case of the fed-batch operation of Escherichia coli for the production of recombinant proteins, it is important to maintai...

media and specific growth rate selection for high-cell-density cultivation of recombinant escherichia coli producing hGM-CSF in fed-batch process

Optimal Feeding Trajectories Design for E. coli Fed-batch Fermentations

In this paper optimal control algorithms for two E. coli fed-batch fermentations are developed. Fed-batch fermentation processes of E. coli strain MC4110 and E. coli strain BL21(DE3)pPhyt109 are considered. Simple material balance models are used to describe the E. coli fermentation processes. The optimal feed rate control of a primary metabolite process is studied and a biomass production is u...

Physiological and Morphological Changes of Recombinant E. coli During Over-Expression of Human Interferon-g in HCDC

The objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant E. coli. Batch and fed-batch culture techniques were employed to grow Escherichia coli BL21 for production of human gamma-interferon in pET expression system. Final cell densities in batch and fed-batch...